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Methods in Molecular Biology Ser.: Organelle Proteomics by Jean Rossier...

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Subject Area
Science
ISBN
9781588297792
Publication Name
Organelle Proteomics
Publisher
Humana Press
Item Length
9.3 in
Subject
Life Sciences / Molecular Biology, Life Sciences / Cell Biology, Life Sciences / Biochemistry, Chemistry / Organic
Publication Year
2008
Series
Methods in Molecular Biology Ser.
Type
Textbook
Format
Hardcover
Language
English
Item Height
0.4 in
Author
Jean Rossier
Item Weight
28.1 Oz
Item Width
6.1 in
Number of Pages
Xvii, 421 Pages

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Product Information

Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study.

Product Identifiers

Publisher
Humana Press
ISBN-10
1588297799
ISBN-13
9781588297792
eBay Product ID (ePID)
63358323

Product Key Features

Number of Pages
Xvii, 421 Pages
Language
English
Publication Name
Organelle Proteomics
Publication Year
2008
Subject
Life Sciences / Molecular Biology, Life Sciences / Cell Biology, Life Sciences / Biochemistry, Chemistry / Organic
Type
Textbook
Subject Area
Science
Author
Jean Rossier
Series
Methods in Molecular Biology Ser.
Format
Hardcover

Dimensions

Item Height
0.4 in
Item Weight
28.1 Oz
Item Length
9.3 in
Item Width
6.1 in

Additional Product Features

Intended Audience
Scholarly & Professional
Dewey Edition
22
Series Volume Number
432
Number of Volumes
1 Vol.
Illustrated
Yes
Dewey Decimal
571.65
Lc Classification Number
Qd415-436
Table of Content
Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography-Tandem Mass Spectrometry.- Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography-Tandem Mass Spectrometry.- Analysis of Organelles, Vesicles, and Protein Machineries.- Purification and Proteomic Analysis of Chloroplasts and their Sub-Organellar Compartments.- Proteomic Analysis of the Stacked Golgi Complex.- Purification of Saccharomyces cerevisiae Mitochondria by Zone Electrophoresis in a Free Flow Device.- Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitochondria.- Mitochondrial Proteomics.- Purification and Proteomic Analysis of the Mouse Liver Mitochondrial Inner Membrane.- Subcellular Fractionation and Proteomics of Nuclear Envelopes.- Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction.- Preparation Methods of Human Metaphase Chromosomes for their Proteome Analysis.- Purification and Proteomic Analysis of Plant Plasma Membranes.- Protocol to Enrich and Analyze Plasma Membrane Proteins from Frozen Tissues.- Proteomic Characterization of Membrane Protein Topology.- Free Flow Isoelectric Focusing.- Use of Gas-Phase Fractionation to Increase Protein Identifications.- Purification and Proteomic Analysis of Lysosomal Integral Membrane Proteins.- Affinity Purification of Soluble Lysosomal Proteins for Mass Spectrometric Identification.- Purification and Proteomic Analysis of Synaptic Vesicles.- Purification and Proteomics Analysis of Pancreatic Zymogen Granule Membranes.- Isolation and Proteomic Analysis of Chlamydomonas Centrioles.- Purification and Proteomic Analysis of 20S Proteasomes from Human Cells.- Characterization of E. coli Ribosomal Particles.- Cellular, Genetic and Molecular Mechanisms of PlantEmbryogenesis.- Assessment of Organelle Purity Using Antibodies and Specific Assays.- Identifying Bona Fide Components of an Organelle by Isotope-Coded Labeling of Subcellular Fractions.- Determination of Genuine Residents of Plant Endomembrane Organelles using Isotope Tagging and Multivariate Statistics.- Quantitative Proteomic Analysis to Profile Dynamic Changes in the Spatial Distribution of Cellular Proteins.- Use of Transcriptomic Data to Support Organelle Proteomic Analysis.
Copyright Date
2008

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