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Pulsed-Field Gel Electrophoresis: Protocols, Methods, and Theories by Margit Bur

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ISBN-13
9781489943910
Book Title
Pulsed-Field Gel Electrophoresis
ISBN
9781489943910
Subject Area
Science
Publication Name
Pulsed-Field Gel Electrophoresis : Protocols, Methods, and Theories
Publisher
Humana Press
Item Length
9 in
Subject
Life Sciences / Biochemistry
Publication Year
2013
Series
Methods in Molecular Biology Ser.
Type
Textbook
Format
Trade Paperback
Language
English
Author
Margit Burmeister, Levy Ulanovsky
Item Weight
25.4 Oz
Item Width
6 in
Number of Pages
Xiii, 481 Pages

關於產品

Product Information

e1ectr0ph0re515 (PF6E) wa51nvented 6are1y 10 year5 a90, 6ut ha5 a1ready 6ec0me a maj0r techn14ue m01ecu- 610109y, 1ar w1th hundred5 0f 0ften the ca5e w1th new techn14ue5 the1r 5ta9e5, m05t pu611cat10n5 are 0- dated 6y the t1me they reach pr1nt. F0r rea50n, a num6er 0fthe auth0r5 0f 600k have effect1ve1y 5erved ph0ne c0n5u1tant5 0n PF6E app11cat10n5 f0r 5evera1 year5. 8y n0w, many 0f the maj0r meth0d5 have 6een te5ted and 1mpr0ved that we fee1 a 600k 0f techn14ue5 W0ve Dur1n9 the prepara- Meth0d5, and 7~, tw0 0ther 600k5 rev1ew1n9 PF6E were pu6115hed. H0wever, the pre5ent w0rk the f1r5t t0 1nc0rp0rate the pr0t0c01 5ty1e, the ha11mark 0f the Meth0d51n that ha5 made that appr0ach the pa5t. 1n add1t10n, many 0f the chapter51nc1ude 0n he1pfu11n 5trate91e5 p1ann1n9 exper1ment5. 7he part 0f the 600k c0nta1n5 the0r1e5 and 065ervat10n5 e1uc1dat1n9 the c0mp1ex 6ehav10r 0f DNA dur1n9 PF6E. F0r any 91ven app11cat10n 0f PF6E, the reader p- ceed 6y a a55em611n9 pr0t0c01 fr0m m0re than 0ne chapter (e. 9. , CHEF, preparat10n 0f a9ar05e 610ck5, and mapp1n9 5trate91e5). 70 he1p pr0ce55, th15 we have tr1ed hard t0 pr0v1de an 1ndex and cr055-reference5 that ea5y acce55 t0 the meth0d5. 1t may 6e u5efu1 t0 read chapter5 n0t 1mmed1ate1y re1ated t0 the cat10n m1nd. F0r examp1e, a u5er 0f CHEF may f1nd he1pfu1 h1nt51n the chapter5 0n F16E, 0FA6E, 0r 7AFE. Certa1n meth0d5, 5uch a5 the preparat10n 0f yea5t marker5, are d0ne 50mewhat d1fferent1y d1fferent 1a65, and the reader f1nd 5evera1 pr0- fr0m wh1ch t0 ch005e.

Product Identifiers

Publisher
Humana Press
ISBN-10
1489943919
ISBN-13
9781489943910
eBay Product ID (ePID)
203431355

Product Key Features

Number of Pages
Xiii, 481 Pages
Language
English
Publication Name
Pulsed-Field Gel Electrophoresis : Protocols, Methods, and Theories
Publication Year
2013
Subject
Life Sciences / Biochemistry
Type
Textbook
Subject Area
Science
Author
Margit Burmeister, Levy Ulanovsky
Series
Methods in Molecular Biology Ser.
Format
Trade Paperback

Dimensions

Item Weight
25.4 Oz
Item Length
9 in
Item Width
6 in

Additional Product Features

Intended Audience
Scholarly & Professional
Reviews
...an excellent reference and experimental manual...a must for every biochemistry and molecular biology library.-Journal of the American Chemical Society
Series Volume Number
12
Number of Volumes
1 Vol.
Illustrated
Yes
Lc Classification Number
Qd415-436
Table of Content
Electrophoretic Techniques.- Field-Inversion Gel Electrophoresis.- Resolving Multimegabase DNA Molecules Using Contour-Clamped Homogeneous Electric Fields (CHEF).- Separation and Size Determination of DNA over a 10-,200 kbp Range.- PFGE Using Double-Inhomogeneous Fields or Orthogonal Field-Alternating Gel Electrophoresis (OFAGE).- Transverse Alternating-Field Electrophoresis.- Rotating Field Gel Electrophoresis (ROFE).- Preparation, Manipulation, and Pulse Strategy for One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE).- Auxiliary Methods.- Preparation, Restriction, and Hybridization Analysis of Mammalian Genomic DNA for Pulsed-Field Gel Electrophoresis.- Encapsulation of Cells in Agarose Beads.- Preparation and Separation of Intact Chromosomes of Vertebrates by One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE).- Isolation of Plant DNA for Pulsed-Field Gel Electrophoresis.- Methyltransferases as Tools to Alter the Specificity of Restriction Endonucleases.- Desktop Digital Imaging.- Biological Applications.- Bacterial Genome Mapping by Two-Dimensional Pulsed-Field Gel Electrophoresis (2D-PFGE).- Protozoan Genomes.- Construction of Yeast Artificial Chromosome Libraries by Pulsed-Field Gel Electrophoresis.- Analysis of Yeast Artificial Chromosome Clones.- Strategies for Mapping Large Regions of Mammalian Genomes.- Two-Dimensional DNA Electrophoresis (2D-DE) for Mammalian DNA.- Pulsed-Field and Two-Dimensional Gel Electrophoresis of Long Arrays of Tandemly Repeated DNA.- Construction of Lambda Libraries from Large PFGE Fragments.- In-Gel Detection of DNA.- Theories and Observations.- Theories of Pulsed-Field Gel Electrophoresis.- The Dynamics of DNA Gel Electrophoresis.- Orientation of the Agarose Matrix by Pulsed Electric Fields.- Reptation Theory ofPulsed Electrophoresis and Trapping Electrophoresis.- Elastic Bag Model of One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE).
Copyright Date
1992

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